# Experimental determination of Fe isotope effect for reduction from Fe(III) to Fe(II) # PI: Seth John # Version: 2019-05-09 ========================= Sample_ID Buffer pH Reductant Reductant_conc Temp Replicate d56Fe err Dd56Fe ------------------------- EAB 1R TRIS 7.05 Ascorbic acid 0.001 2 1 -1.83 0.03 -2.18 EAB 2R TRIS 7.05 Ascorbic acid 0.001 2 2 -2.59 0.03 -2.94 EAB 3R TRIS 7.05 Ascorbic acid 0.001 2 3 -2.33 0.03 -2.68 EAB 4R TRIS 7.05 Ascorbic acid 0.001 23 1 -1.9 0.03 -2.25 EAB 5R TRIS 7.05 Ascorbic acid 0.001 23 2 -2.1 0.03 -2.45 EAB 6R TRIS 7.05 Ascorbic acid 0.001 23 3 -2.43 0.04 -2.78 EAB 7R TRIS 7.05 Ascorbic acid 0.0001 23 1 -1.53 0.02 -1.88 EAB 8R TRIS 7.05 Ascorbic acid 0.0001 23 2 -0.93 0.02 -1.28 EAB 9R TRIS 7.05 Ascorbic acid 0.0001 23 3 -1.59 0.03 -1.94 EAB 10R TRIS 7.05 Ascorbic acid 0.0001 60 1 -2.27 0.04 -2.62 EAB 11R TRIS 7.05 Ascorbic acid 0.0001 60 2 -0.51 0.02 -0.86 EAB 12R TRIS 7.05 Ascorbic acid 0.0001 60 3 -1.11 0.02 -1.46 EAB 13R TRIS 7.05 Hydroxylamine hydrochloride 0.001 23 1 -2.53 0.03 -2.88 EAB 14R TRIS 7.05 Hydroxylamine hydrochloride 0.001 23 2 -3.09 0.02 -3.44 EAB 15R TRIS 7.05 Hydroxylamine hydrochloride 0.001 23 3 -2.17 0.04 -2.52 EAB 16R TRIS 7.05 Sodium Dithionite 0.001 23 1 -0.54 0.03 -0.89 EAB 17R TRIS 7.05 Sodium Dithionite 0.001 23 2 -0.72 0.03 -1.07 EAB 18R TRIS 7.05 Sodium Dithionite 0.001 23 3 -0.54 0.04 -0.89 EAB 19R TRIS 7.05 Hydroxylamine hydrochloride 0.001 2 1 -3.82 0.03 -4.17 EAB 20R TRIS 7.05 Hydroxylamine hydrochloride 0.001 2 2 -3.48 0.03 -3.83 EAB 21 TRIS 7.05 Hydroxylamine hydrochloride 0.001 2 3 -3.21 0.03 -3.56 EAB 22 TRIS 7.05 Hydroxylamine hydrochloride 0.001 60 1 -2.57 0.03 -2.92 EAB 23 TRIS 7.05 Hydroxylamine hydrochloride 0.001 60 2 -2.73 0.03 -3.08 EAB 24 TRIS 7.05 Hydroxylamine hydrochloride 0.001 60 3 -3.12 0.03 -3.47 EAB 25 TRIS 7.05 Hydroxylamine hydrochloride 0.001 100 1 -2.93 0.04 -3.28 EAB 26 TRIS 7.05 Hydroxylamine hydrochloride 0.001 100 2 -2.91 0.03 -3.26 EAB 27 TRIS 7.05 Hydroxylamine hydrochloride 0.001 100 3 -2.66 0.04 -3.01 EAB 28 TRIS 7.05 Sodium Dithionite 0.001 2 1 -0.02 0.02 -0.37 EAB 29 TRIS 7.05 Sodium Dithionite 0.001 2 2 0.21 0.02 -0.14 EAB 30 TRIS 7.05 Sodium Dithionite 0.001 2 3 0.26 0.02 -0.09 EAB 31 TRIS 7.05 Sodium Dithionite 0.001 60 1 -1.86 0.03 -2.21 EAB 32 TRIS 7.05 Sodium Dithionite 0.001 60 2 -1.7 0.03 -2.05 EAB 33 TRIS 7.05 Sodium Dithionite 0.001 60 3 -1.49 0.03 -1.84 EAB 34 TRIS 7.05 Sodium Dithionite 0.001 100 1 2.95 0.03 2.6 EAB 35 TRIS 7.05 Sodium Dithionite 0.001 100 2 3.05 0.03 2.7 EAB 36 TRIS 7.05 Sodium Dithionite 0.001 100 3 2.98 0.04 2.63 EAB 37 TRIS 7.05 Ascorbic acid 0.0001 2 1 -0.94 0.03 -1.29 EAB 38 TRIS 7.05 Ascorbic acid 0.0001 2 2 -0.83 0.03 -1.18 EAB 39 TRIS 7.05 Ascorbic acid 0.0001 2 3 -0.69 0.03 -1.04 EAB 40 TRIS 7.05 Manganese sulfate 0.001 2 1 -0.17 0.02 -0.52 EAB 41 TRIS 7.05 Manganese sulfate 0.001 2 2 -0.39 0.02 -0.74 EAB 42 TRIS 7.05 Manganese sulfate 0.001 2 3 -0.34 0.01 -0.69 EAB 43 TRIS 7.05 Manganese sulfate 0.001 23 1 -0.09 0.02 -0.44 EAB 44 TRIS 7.05 Manganese sulfate 0.001 23 2 -0.08 0.02 -0.43 EAB 45 TRIS 7.05 Manganese sulfate 0.001 23 3 -0.19 0.03 -0.54 EAB 46 TRIS 7.05 Ascorbic acid 1e-05 100 1 3.84 0.02 3.49 EAB 47 TRIS 7.05 Ascorbic acid 1e-05 100 2 3.69 0.02 3.34 EAB 48 TRIS 7.05 Ascorbic acid 1e-05 100 3 3.96 0.02 3.61 EAB 49 TRIS 7.05 Manganese sulfate 0.001 60 1 0.36 0.02 0.01 EAB 50 TRIS 7.05 Manganese sulfate 0.001 60 2 0.27 0.01 -0.08 EAB 51 TRIS 7.05 Manganese sulfate 0.001 60 3 0.34 0.02 -0.01 EAB 52 TRIS 7.05 Manganese sulfate 0.001 100 1 3.79 0.02 3.44 EAB 53 TRIS 7.05 Manganese sulfate 0.001 100 2 3.95 0.02 3.6 EAB 54 TRIS 7.05 Manganese sulfate 0.001 100 3 3.89 0.02 3.54 EAB 55 TRIS 7.05 sunlight+EDTA 0.001 23 1 5.37 0.02 5.02 EAB 56 TRIS 7.05 sunlight+EDTA 0.001 23 2 5.74 0.03 5.39 EAB 57 TRIS 7.05 sunlight+EDTA 0.001 23 3 5.42 0.03 5.07 EAB 58 TRIS 7.05 sunlight+Glucaric acid 0.001 23 1 0.18 0.02 -0.17 EAB 59 TRIS 7.05 sunlight+Glucaric acid 0.001 23 2 0.19 0.03 -0.16 EAB 60 TRIS 7.05 sunlight+Glucaric acid 0.001 23 3 0.05 0.04 -0.3 EAB 61 MES 5.04 Ascorbic acid 0.0001 23 1 -3.46 0.02 -3.81 EAB 62 MES 5.04 Ascorbic acid 0.0001 23 2 -3.59 0.03 -3.94 EAB 63 MES 5.04 Ascorbic acid 0.0001 23 3 -3.34 0.03 -3.69 EAB 64 MES 6.01 Ascorbic acid 0.0001 23 1 -3.66 0.02 -4.01 EAB 65 MES 6.01 Ascorbic acid 0.0001 23 2 -3.82 0.03 -4.17 EAB 66 MES 6.01 Ascorbic acid 0.0001 23 3 -3.09 0.02 -3.44 EAB 67 MES 7.05 Ascorbic acid 0.0001 23 1 -3.14 0.02 -3.49 EAB 68 MES 7.05 Ascorbic acid 0.0001 23 2 -3.1 0.04 -3.45 EAB 69 MES 7.05 Ascorbic acid 0.0001 23 3 -2.91 0.03 -3.26 EAB 70 TRIS 7.05 Ascorbic acid 0.0001 23 1 -0.88 0.02 -1.23 EAB 71 TRIS 7.05 Ascorbic acid 0.0001 23 2 -1.88 0.02 -2.23 EAB 72 TRIS 7.05 Ascorbic acid 0.0001 23 3 -1.61 0.02 -1.96 EAB 73 TRIS 8.08 Ascorbic acid 0.0001 23 1 -0.63 0.02 -0.98 EAB 74 TRIS 8.08 Ascorbic acid 0.0001 23 2 -0.7 0.03 -1.05 EAB 75 TRIS 8.08 Ascorbic acid 0.0001 23 3 -0.72 0.02 -1.07 EAB 76 TRIS 9.02 Ascorbic acid 0.0001 23 1 -2.48 0.03 -2.83 EAB 77 TRIS 9.02 Ascorbic acid 0.0001 23 2 -2.87 0.03 -3.22 EAB 78 TRIS 9.02 Ascorbic acid 0.0001 23 3 -3.27 0.03 -3.62 EAB 79 MES 5.04 sunlight+EDTA 0.001 23 1 1.85 0.04 1.5 EAB 80 MES 5.04 sunlight+EDTA 0.001 23 2 1.81 0.02 1.46 EAB 81 MES 5.04 sunlight+EDTA 0.001 23 3 2.53 0.03 2.18 EAB 82 MES 6.01 sunlight+EDTA 0.001 23 1 1.32 0.03 0.97 EAB 83 MES 6.01 sunlight+EDTA 0.001 23 2 1.74 0.02 1.39 EAB 84 MES 6.01 sunlight+EDTA 0.001 23 3 2.02 0.03 1.67 EAB 85 MES 7.05 sunlight+EDTA 0.001 23 1 1.99 0.02 1.64 EAB 86 MES 7.05 sunlight+EDTA 0.001 23 2 0.84 0.03 0.49 EAB 87 MES 7.05 sunlight+EDTA 0.001 23 3 1.7 0.02 1.35 EAB 88 TRIS 8.08 sunlight+EDTA 0.001 23 1 2.07 0.02 1.72 EAB 89 TRIS 8.08 sunlight+EDTA 0.001 23 2 1.28 0.02 0.93 EAB 90 TRIS 8.08 sunlight+EDTA 0.001 23 3 1.43 0.03 1.08 EAB 91 TRIS 7.05 sunlight+EDTA 0.001 23 1 5.41 0.02 5.06 EAB 92 TRIS 7.05 sunlight+EDTA 0.001 23 2 4.9 0.02 4.55 EAB 93 TRIS 7.05 sunlight+EDTA 0.001 23 3 5.66 0.02 5.31 EAB 94 TRIS 9.02 sunlight+EDTA 0.001 23 1 -0.7 0.05 -1.05 EAB 95 TRIS 9.02 sunlight+EDTA 0.001 23 2 -0.66 0.04 -1.01 EAB 96 TRIS 9.02 sunlight+EDTA 0.001 23 3 -0.37 0.02 -0.72 EAB 97 TRIS 8.08 incubator+EDTA 0.001 23 1 -1.8 0.02 -2.15 EAB 98 TRIS 8.08 incubator+EDTA 0.001 23 2 -1.84 0.03 -2.19 EAB 99 TRIS 8.08 incubator+EDTA 0.001 23 3 -1.6 0.03 -1.95